PlasmidsX

Plasmid editor · iPhone, iPad and Mac

Every construct, drawn clearly.

Open a GenBank or SnapGene file and PlasmidsX draws the map, finds the cut sites and lays out every base with its annotations. Plan a digest or a Gibson assembly before you go to the bench.

Download on the App Store Free to view, import and analyze.
Editing and cloning with PlasmidsX Pro.
Circular map of pEGFP-N1, 4,733 bp, with its features and unique restriction sites
pEGFP-N14,733 bp · circular
588
commercial enzymes from REBASE
300 kb
sequences that still scroll smoothly
5
file formats: GenBank, FASTA, SnapGene, ApE, PlasmidsX
7
languages, from German to Japanese

Sequence

Every base, with what it means.

Both strands, the translation and the cut sites line up base for base. Here is the multiple cloning site of pEGFP-N1, right before the EGFP start codon.

586 pEGFP-N1 · MCS ACGT
Edit

A keyboard for DNA

Type, paste and cut bases. Features stretch and shrink with your edits, and you're warned when an edit breaks a coding sequence.

Primers

Primers that know their Tm

Melting temperatures from SantaLucia nearest-neighbor values, with salt and Mg²⁺ corrections. Keep a shared primer library.

Find

Search inside sequences

Find a motif on either strand, or jump straight to a position. Search also reaches into every plasmid in your library.

Digest & Gel

Run the gel before you pour it.

Choose enzymes and PlasmidsX cuts the plasmid and runs the fragments next to a real ladder. Sites blocked by Dam or Dcm methylation are flagged, so a missing band doesn't surprise you.

Virtual 1% agarose gel of pEGFP-N1 digests next to the 1 kb Plus ladder
1% agarose · 100 V · 0 min

Cloning · Pro

Assemble it on screen first.

Cut the vector, add an insert and watch the junctions form. Annotations carry across into the product, and the result keeps a record of where it came from.

Restriction & ligationGibson / HiFiGolden GateGatewayPCR
Gibson assembly: an mCherry insert with 20 bp overlaps joins the NheI and NotI cut pEGFP-N1 vector
1 · Fragments2 · 5′ exonuclease3 · Anneal4 · Fill & seal

On iPhone

Five tabs for one plasmid.

Map, Features, Enzymes, Sequence and Tools. Scroll sideways to see them.

iPad and Mac

Room for the whole construct.

On iPad and Mac the library, the map and an inspector sit side by side. Select a feature, a primer or a cut site and its details and actions are right there.

  • Several plasmids open at once, each in its own tab or window
  • Drag and drop files in and out, keyboard shortcuts and Apple Pencil
  • Your library syncs through iCloud, with a Home Screen widget
PlasmidsX on iPad: the library, the map and the inspector side by side

Plans

Free to explore. Pro when you build.

Viewing, importing, annotating and analyzing are free. A PlasmidsX Pro subscription adds the tools for changing and building constructs.

PlasmidsX

Free

  • Circular and linear maps, sequence view
  • Features, primers and a shared primer library
  • Restriction analysis, ORFs, Digest & Gel
  • Import every supported format; FASTA and image export; print
  • Saved versions and the Home Screen widget

PlasmidsX Pro PRO

Monthly or yearly subscription

  • Sequence editing: type, paste, cut, reverse complement, set the origin
  • Cloning: restriction and ligation, Gibson Assembly, Golden Gate, Gateway and PCR
  • Alignment of sequencing reads to your plasmid
  • BLAST searches and sequence downloads from NCBI
  • GenBank and SnapGene export

PlasmidsX Pro is an auto-renewing subscription, billed monthly or yearly. Payment is charged to your Apple Account when you confirm the purchase. The subscription renews automatically unless you cancel it at least 24 hours before the end of the current period. You can manage or cancel your subscription in your Apple Account settings. Prices are shown in the App Store.

Privacy

Your sequences stay yours.

No account, no ads and no tracking. Your plasmids stay on your device and in your own iCloud.

No account

Open the app and start working. There is nothing to sign up for.

No ads, no tracking

PlasmidsX doesn't show ads and doesn't track you across apps or websites.

Only when you ask

A BLAST search or an NCBI download sends NCBI only what it needs, and only when you start it.

Read the full privacy policy.

Take your plasmids with you.

PlasmidsX for iPhone, iPad and Mac.

Download on the App Store